Parallax: High Accuracy Three-Dimensional Single Molecule Tracking Using Split Images
- 4 June 2009
- journal article
- letter
- Published by American Chemical Society (ACS) in Nano Letters
- Vol. 9 (7) , 2676-2682
- https://doi.org/10.1021/nl901129j
Abstract
Three-dimensional (3D) tracking can provide valuable biological insights that are missing in conventional microscopy. Here we developed a single molecule 3D tracking microscopy technique, named Parallax, with high localization precision and temporal resolution. We demonstrated its capabilities by studying the 3D trafficking of glucose-transporter-4 containing vesicles in living adipocytes as well as the walking path of single myosin VI molecules along actin filaments.Keywords
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