An Investigation into the Effect of Reagent Volume on the Spin Indirect Antiglobulin Test

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Abstract
Three polyspecific antiglobulin reagents were tested in a spin antiglobulin technique with 51 antibodies of varying blood group specificities in which the vol of antiglobulin reagent used ranged from 1-3 vol (35-105 .mu.l). Statistical analysis of results showed that a significant decrease in avidity and agglutination scores occurred as the volume of antiglobulin reagent used was increased (vol effect). The vol effect was shown by 33/51 (65%) of antibodies with all 3 antiglobulin reagents and by 48/51 (94%) of antibodies with at least 1 antiglobulin reagent. Only 3/51 (6%) failed to show the vol effect with all 3 antiglobulin reagents.

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