Experimental intra-ovum infection of coho salmon (Oncorhynchus kisutch) eggs with Renibacterium salmoninarum using a microinjection technique

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Abstract
Renibacterium salmoninarum the causative agent of bacterial kidney disease in salmonids is transmitted from parent to progeny via the egg. Natural intra-ovum infection rates are low, making it necesary to use large samples sizes when attempting any conclusions on the efficacy of treatments to eliminate these infections. To obviate this problem, a microinjection technique was developed for inducing intra-ovum infection at high prevalence. The technique, described in this paper, required the use of relatively inexpensive equipment, resulted in good egg survival rates (73% to the eyed stage), and ensured high intra-ovum infection rates: 91 and 82% at the eyed and alevin stages, respectively.

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