Early effects of Phytohemagglutinin on induction of DNA polymerase, thymidine kinase, deoxyribonucleoside triphosphate pools and DNA synthesis in human lymphocytes
Open Access
- 1 August 1977
- journal article
- research article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 4 (8) , 2713-2724
- https://doi.org/10.1093/nar/4.8.2713
Abstract
In Phytohemagglutinin stimulated human lymphocytes the time relationship was determined between induction of the parameters mentioned. The results indicate that the induction occurred in a specific sequence. Thus, a simultaneous increase in the activity of DNA polymerase and thymidinekinase occurred after 15 h of incubation with Phytohemagglutinin. Furthermore, this enhancement occurred 2 h before the expansion of the TTP and dCTP pools and 4 h before the expansion of the dATP and dGTP pools. The rate of [ 3 H] deoxyguanosine incorporation into DNA increased simultaneously with the expansion of the TTP and dCTP pools.Keywords
This publication has 20 references indexed in Scilit:
- The pool size of deoxyguanosine 5′-triphosphate and deoxycytidine 5′-triphosphate in phytohemagglutinin-stimulated and non-stimulated human lymphocytesExperimental Cell Research, 1975
- Cell-cycle-dependent variations of deoxyribonucleoside triphosphate pools in chinese hamster cellsBiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1973
- The deoxyribonucleoside 5′-triphosphate (dATP and dTTP) pool in phytohemagglutinin-stimulated and non-stimulated human lymphocytesExperimental Cell Research, 1973
- DNA polymerase activity in phytohemagglutinin-stimulated and non-stimulated human lymphocytesExperimental Cell Research, 1973
- Deoxyribonucleoside‐Triphosphate Pools and DNA Synthesis in Synchronized Hamster CellsEuropean Journal of Biochemistry, 1973
- Deoxyribonucleoside 5′-triphosphate pool fluctuations during the mamalian cell cycleBiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1972
- Thymidine kinase, deoxycytidine kinase and deoxycytidylate deaminase activities in phytohaemagglutinin stimulated human lymphocytesExperimental Cell Research, 1971
- Deoxyribonucleoside triphosphate pools in synchronized and drug-inhibited L929 cellsBiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1971
- DNA polymerase: Correlation with DNA replication during transformation of human lymphocytesExperimental Cell Research, 1971
- DNA polymerase activity of cultured normal and leukemic lymphocytes: Response to phytohemagglutininExperimental Cell Research, 1969