Glycosphingolipid Biosynthesis through Asialogangliosides in Rat Bone Marrow Cells1

Publisher Website
Google Scholar
Abstract
Glycolipid biosynthesis in rat bone marrow cells has been studied with reference to four kinds of glycosyltransferases catalyzing the transfer of TV-acetylgalactosamine, galactose, N -acetylneuraminic acid, and fucose to each glycolipid acceptor. It was demonstrated that glycosyltransferase activities which synthesize galactosylglucosyl-ceramide (CDH) from glucosylceramide (CMH), . N -acetylgalactosaminylgalactosyl-glucosylceramide (GA 2 ) from CDH, galactosyl- N -acetylgalactosaminylgalactosyl-glucosylceramide (GA 1 ) from GA 2 and N -acetylneuraminylgaIactosy]-Af-acetyl-galactosaminylgalactosylglucosylceramide (GM 1 ) from GA 1 were all present in rat bone marrow cell homogenate. Fucosyltransferase activity catalyzing the transfer of fucose from GDP-fucose to GA 1 was also recognized in the cell homogenate. Neutral glycolipid extracted from rat bone marrow cells was analyzed by thin layer chromatography and glycosidase treatments. The presence of glycolipids corresponding to GA 2 , GA 1 and fucolipid was demonstrated. From these results, it was concluded that the biosynthesis of glycolipid through asialogangliosides is a major biosynthetic route in rat bone marrow cells.