crpX mutants of Escherichia coli K12: Specific regulatory effects of altered cyclic AMP receptor proteins
- 1 October 1982
- journal article
- research article
- Published by Springer Nature in Molecular Genetics and Genomics
- Vol. 187 (2) , 291-296
- https://doi.org/10.1007/bf00331131
Abstract
We attempted to correlate structural modifications of the adenosine 3′,5′ cyclic monophosphate (cAMP) receptor protein (CAP), to changes in some of its in vivo regulatory functions such as (i) stimulation of the lactose operon expression and (ii) control of adenylate cyclase activity. A radioimmunological procedure was used to study the structure of CAP synthesized by three mutants (crpX) grown under various conditions, in the presence or absence of endogenous or exogenous cAMP. In one mutant CAP appears to be sensitive to thermal inactivation. In another mutant CAP is particularly sensitive to degradation in the absence of cAMP; this degradation is enhanced by high temperature and during stationary phase of grwoth, and prevented by the addition of glucose. Functional alterations of CAP were not found to follow structural changes strictly. In the crpX mutants and in strains carrying the crp + or other crp allele, the stimulation of the lactose operon expression and the modulation of the in vivo rates of cAMP synthesis appear to vary in parallel, favoring an indirect mechanism of regulation of adenylate cyclase by CAP.This publication has 20 references indexed in Scilit:
- Multiple regulation of the activity of adenylate cyclase in Escherichia coliMolecular Genetics and Genomics, 1982
- Regulation of the synthesis of adenylate cyclase in Escherichia coli by the cAMP — cAMP receptor protein complexMolecular Genetics and Genomics, 1981
- CrpXmutants ofEscherichia coliK-12: Selection and physiological propertiesFEMS Microbiology Letters, 1981
- Regulatory aspects of the cyclic AMP receptor protein in Escherichia coli K-12Biochemical and Biophysical Research Communications, 1980
- Involvement of cyclic AMP and its receptor protein in the sensitivity of Escherichia coli K 12 toward serineMolecular Genetics and Genomics, 1979
- Determination of the rates of synthesis and degradation of adenosine 3′,5′-cyclic monophosphate in Escherichia coli CRP− and CRP+ strainsCanadian Journal of Biochemistry, 1978
- The cyclic 3′,5′-adenosine monophosphate receptor protein and regulation of cyclic 3′,5′-adenosine monophosphate synthesis in Escherichia coliMolecular Genetics and Genomics, 1978
- A simple ultrasensitive method for the assay of cyclic AMP and cyclic GMP in tissuesLife Sciences, 1976
- Abnormally high rate of cyclic AMP excretion from an Escherichia coli mutant deficient in cyclic AMP receptor proteinBiochemical and Biophysical Research Communications, 1974
- The genetic control and cytoplasmic expression of “Inducibility” in the synthesis of β-galactosidase by E. coliJournal of Molecular Biology, 1959