A TECHNIQUE FOR THE STUDY OF ACETYLCHOLINE TURNOVER IN MOUSE BRAIN IN VIVO
- 1 May 1969
- journal article
- Published by Wiley in Journal of Neurochemistry
- Vol. 16 (5) , 695-700
- https://doi.org/10.1111/j.1471-4159.1969.tb06447.x
Abstract
Abstract— —A method to measure the rate of acetylcholine turnover in mouse brain in vivo has been developed. It is based on the formation of labelled acetylcholine from intravenously injected labelled choline. The isotopic dilution of choline in the brain has been measured by assaying endogenous choline in the brain by an enzymatic method using tritium‐labelled acetyl‐CoA and purified choline acetyltransferase.The rate of acetylcholine turnover in the brain could be calculated at 50 n‐moles acetylcholine/g/min in conscious mice. In anaesthetized mice and in mice treated with oxotremorine, a decrease of acetylcholine turnover to about 10 n‐moles/g/min was found.Keywords
This publication has 18 references indexed in Scilit:
- Choline acetyltransferase inhibitors: a group of styryl-pyridine analogsBiochemical Pharmacology, 1967
- 3-Bromoacetonyltrimethylammonium Bromide, a Choline Acetylase Inhibitor.Acta Chemica Scandinavica, 1967
- Choline acetyltransferase purification and effect of salts on the mechanism of the enzyme-catalyzed reactionBiochimica et Biophysica Acta (BBA) - Enzymology and Biological Oxidation, 1966
- ACETYLCOENZYME A IN BRAIN THE EFFECT OF CENTRALLY ACTIVE DRUGS, INSULIN COMA AND HYPOXIAJournal of Neurochemistry, 1966
- UPTAKE OF CHOLINE BY MOUSE BRAIN SLICES*Journal of Neurochemistry, 1966
- ACETYLCHOLINE RELEASE FROM THE BRAINPublished by Elsevier ,1966
- Effect of ‘Tremorine’ and some Anti-Parkinson's Disease Drugs on Acetylcholine in the Rat's BrainNature, 1963
- Tremorine and atropine effects on brain acetylcholineLife Sciences, 1963
- Increase in liver acetyl — Coenzyme a during ketosisBiochemical and Biophysical Research Communications, 1963
- DRUG‐INDUCED CHANGES IN BRAIN ACETYLCHOLINEBritish Journal of Pharmacology and Chemotherapy, 1962