Computerized analyzing system for chemiluminescence.
- 1 January 1984
- journal article
- research article
- Published by Pharmaceutical Society of Japan in CHEMICAL & PHARMACEUTICAL BULLETIN
- Vol. 32 (8) , 3079-3087
- https://doi.org/10.1248/cpb.32.3079
Abstract
A pneumatically-driven injection device and a computer analyzing system for chemiluminescence studies was made. This total instrument provided superior reproducibilities, e.g., within-run variabilities (n = 4-6) of entire light emission were about 1-4%. The entire emission is calculated from both an integration of the signal and an approximation to a first-order reaction. Rapid, intense light (t1/2 [half-time] = 0.35) was produced by luminol reaction catalyzed by concentrated hemin (10-5 M). Applications of the equipment, the analyzing method and hemin as a catalyst are discussed to analytical and clinical chemistry.This publication has 8 references indexed in Scilit:
- Automated bioluminescent assays for NADH, glucose 6-phosphate, primary bile acids, and ATPAnalytical Biochemistry, 1983
- Absorption-corrected chemiluminescence measurements with a dual-pathlength spectrometerAnalytical Chemistry, 1983
- A sensitive method for determination of serum hemoglobin based on iso-luminol chemiluminescenceClinica Chimica Acta; International Journal of Clinical Chemistry, 1982
- Luminescence immunoassay of human serum albumin with hemin as labeling catalystAnalytical Chemistry, 1982
- Immunochemiluminometric assay for hepatitis B surface antigen.Clinical Chemistry, 1981
- A luminol-assisted, competitive-binding immunoassay of human immunoglobulin GAnalytical Biochemistry, 1979
- Chemiluminescence yields and detection limits of some isoluminol derivatives in various oxidation systemsAnalytical Chemistry, 1978
- Chemiluminescence of luminol in the presence of hematin compoundsAnalytical Biochemistry, 1965