Abstract
The rate of formation of isocitrate and cis-aconitate from citrate in the presence of heart-muscle aconitase was measured. Contrary to findings by Friedrich-Freksa and Martius (Zeitschr. Naturforsch. 6b: 296. 1951) the rate of isocitrate formation showed a distinct lag period. The observations are in agreement with Martius''s earlier assumption (Zeitschr. Physiol. Chem. 247: 104. 1937) that cis-aconitate is an intermediate in the conversion of citrate into isocitrate.

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