Dimerization of human immunodeficiency virus type 1 RNA involves sequences located upstream of the splice donor site

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Abstract
The retrovlral genome consists of two homologous RNA molecules associated close to their 5′ ends. We studied the spontaneous dimerization of four HIV-1 RNA fragments (RNAs 1–707, 1–615, 311–612, and 311–415) containing the previously defined dimerization domain, and a RNA fragment (RNA 1–311) corresponding to the upstream sequences. Significant dimerization of all RNAs Is observed on agarose gels when magnesium is Included In the electrophoresls buffer. In contrast to dimerization of RNAs 311–612 and 311–415, dimerization of RNAs 1–707, 1–615 and 1–311 strongly depends on the size of the monovalent cation present in the Incubation buffer. Also, dimerization of RNAs 1–707, 1–615, and 1–311 is 10 times faster than that of RNAs 311–612 and 311–415. The dlmers formed by the latter RNAs are substantially more stable than that of RNA 1–615, while RNA 1–311 dlmer is 5–7°C less stable than RNA 1–615 dimer. These results Indicate that dimerization of HIV-1 genomic RNA Involves elements located upstream of the splice donor site (position 305), i.e. outside of the previously defined dimerization domain.