A highly conserved eukaryotic protein family possessing properties of polypeptide chain release factor
- 1 December 1994
- journal article
- Published by Springer Nature in Nature
- Vol. 372 (6507) , 701-703
- https://doi.org/10.1038/372701a0
Abstract
The termination of protein synthesis in ribosomes is governed by termination (stop) codons in messenger RNAs and by polypeptide chain release factors (RFs). Although the primary structure of prokaryotic RFs and yeast mitochrondrial RF is established, that of the only known eukaryotic RF (eRF) remains obscure. Here we report the assignment of a family of tightly related proteins (designated eRF1) from lower and higher eukaryotes which are structurally and functionally similar to rabbit eRF. Two of these proteins, one from human and the other from Xenopus laevis, have been expressed in yeast and Escherichia coli, respectively, purified and shown to be active in the in vitro RF assay. The other protein of this family, sup45 (sup1) of Saccharomyces cerevisiae, is involved in omnipotent suppression during translation. The amino-acid sequence of the eRF1 family is highly conserved. We conclude that the eRF1 proteins are directly implicated in the termination of translation in eukaryotes.Keywords
This publication has 22 references indexed in Scilit:
- Localization and characterization of the gene encoding release factor RF3 in Escherichia coli.Proceedings of the National Academy of Sciences, 1994
- Identification of the prfC gene, which encodes peptide-chain-release factor 3 of Escherichia coli.Proceedings of the National Academy of Sciences, 1994
- In Xenopus laevis, the product of a developmentally regulated mRNA is structurally and functionally homologous to a Saccharomyces cerevisiae protein involved in translation fidelity.Molecular and Cellular Biology, 1993
- The yeast nuclear geneMRF1encodes a mitochondrial peptide chain release factor and cures several mitochondrial RNA splicing defectsNucleic Acids Research, 1992
- Ribosomal association of the yeast SAL4 (SUP45) gene product: implications for its role in translation fidelity and terminationMolecular Microbiology, 1992
- Identification of a human cDNA with high homology to yeast omnipotent suppressor 45Gene, 1992
- Recent advances in peptide chain terminationMolecular Microbiology, 1990
- Yeast omnipotent supressorSUP1(SUP45): nucleotide sequence of the wildtype and a mutant geneNucleic Acids Research, 1986
- Isolation of the SUP45 omnipotent suppressor gene of Saccharomyces cerevisiae and characterization of its gene product.Molecular and Cellular Biology, 1985
- Mammalian Peptide Chain Termination, II. Codon Specificity and GTPase Activity of Release FactorProceedings of the National Academy of Sciences, 1971