Improved Routine Maintenance of Nippostrongylus brasiliensis Culture

Publisher Website
Google Scholar
Abstract
A new method is described for the in vitro culture of the preparasitic stages of Nippostrongylus brasiliensis. It utilises the plating of faecal suspensions on to paper chromatography strips supported on non-porous Perspex slabs and has been designed primarily to facilitate savings in the time required for routine culture maintenance without lowering the quantity or quality of the larval yield. Comparison with the filter paper/Petri dish method shows an increase in larval yield of 85% and a reduction in time required for culture of 72%. Total inhibition of fungal growth on incubated faeces suspensions was obtained by the addition of “Mycostatin” (Squibb) in a concentration of 62 units per ml. or more.