On the Correlation of S-S and C-S Raman Bands Frequencies and Intensities with Conformations of Disulfide Bridges in Proteins
- 1 August 1985
- journal article
- research article
- Published by Taylor & Francis in Journal of Biomolecular Structure and Dynamics
- Vol. 3 (1) , 185-196
- https://doi.org/10.1080/07391102.1985.10508406
Abstract
We have studied dependence of the S-S and C-S raman bands frequencies and intensities on the conformation of disulfide bridges. These correlations have been analyzed on the basis of the normal coordinates treatment in the valence force field and from comparison of the raman and X-ray data for the model compounds and proteins. The found regularity of the intensity variations with conformation make it possible to distinguish these spectral perturbations from those associated with the change in the number of disulfide bridges in proteins. Particularly, this was demonstrated on the γ-irradiated insulin solutions spectra. We also present some data appropriate for estimation of the content of disulfide bridges in proteins using internal intensity standards.This publication has 20 references indexed in Scilit:
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