Sub-second isoelectric focusing in free flow using a microfluidic device
- 7 October 2003
- journal article
- research article
- Published by Royal Society of Chemistry (RSC) in Lab on a Chip
- Vol. 3 (4) , 224-227
- https://doi.org/10.1039/b308476k
Abstract
Using a microfabricated chip with a bed volume of 0.2 µL we demonstrate the validity of the scaling laws for molecular mass transport of isoelectric focusing (IEF) in free flow. Nano- or microlitre sample volumes can be concentrated within 430 ms by a factor of up to 400. These very fast performances make the chip applicable to proteomic analysis and for continuous monitoring of biochemical processes.Keywords
This publication has 15 references indexed in Scilit:
- High-Speed Free-Flow Electrophoresis on ChipAnalytical Chemistry, 2003
- Isoelectric focusing in long immobilized pH gradient gels to improve protein separation in proteomic analysisElectrophoresis, 2003
- Miniaturization and chip technology. What can we expect?Pure and Applied Chemistry, 2001
- Separation and Purification of Methadone Enantiomers by Continuous- and Interval-Flow ElectrophoresisAnalytical Chemistry, 1999
- Continuous Separation of High Molecular Weight Compounds Using a Microliter Volume Free-Flow Electrophoresis MicrostructureAnalytical Chemistry, 1996
- Free flow‐isoelectric focusing of human cellular lysates as sample preparation for protein analysisElectrophoresis, 1995
- Continuous Sample Pretreatment Using a Free-Flow Electrophoresis Device Integrated onto a Silicon ChipAnalytical Chemistry, 1994
- Micromachining a Miniaturized Capillary Electrophoresis-Based Chemical Analysis System on a ChipScience, 1993
- Conditions for purification of proteins by free‐flow zone electrophoresisElectrophoresis, 1990
- Miniaturized total chemical analysis systems: A novel concept for chemical sensingSensors and Actuators B: Chemical, 1990