Detection of human papillomaviruses from histologically normal lymph nodes of Japanese cervical cancer patients by nested polymerase chain‐reaction assay
- 1 April 1993
- journal article
- research article
- Published by Wiley in International Journal of Cancer
- Vol. 53 (6) , 932-937
- https://doi.org/10.1002/ijc.2910530611
Abstract
To determine the prognostic significance of human papilloma virus (HPV) DNA in histologically normal lymph nodes, we developed a nested polymerase chain‐reaction (PCR) method on HPV16, 18 and 33 DNAs for formalin‐fixed, paraffin‐embedded tissues. We investigated 370 histologically normal lymph nodes from 15 patients treated for stage‐IB/IIB (FIGO) invasive cervical cancer. HPV16 DNA was detected in 7 (47%) and HPV18 DNA in 3 (20%) of the cervical cancers. Examination of histologically normal lymph nodes from these 10 patients by nested PCR revealed HPV DNA in 5 (50%) of them; in all cases HPV type in lymph nodes and tumor was the same. Two of these 5 patients had a recurrence (pelvic cavity or lung) and died of cancer, although all 5 had had pelvic radiotherapy after radical hysterectomy and lymphadenectomy. These findings indicate that nested PCR is useful for evaluating early lymph‐node involvement retrospectively in HPV‐positive cases.Keywords
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