Characterization and linkage mapping often sheep microsatellite markers derived from a sheep x hamster cell hybrid
- 24 April 2009
- journal article
- Published by Wiley in Animal Genetics
- Vol. 27 (3) , 203-206
- https://doi.org/10.1111/j.1365-2052.1996.tb00953.x
Abstract
A cosmid library has been constructed from a sheep x hamster cell hybrid containing sheep chromosome t1, rob (6;24). Clones containing sheep DNA were identified by hybridizing to a total sheep genomic DNA probe. Small fragments (< 500 bp) containing (AC)n microsatellites were subcloned and sequenced. Ten microsatellite markers were characterized and six were mapped back to chromosomes 6 and 24. The remaining microsatellites mapped to chromosome 26, which was shown to be present in a small proportion of cells of the cell line.Keywords
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