The Linamarin β-Glucosidase in Costa Rican Wild Lima Beans (Phaseolus lunatus L.) Is Apoplastic
- 1 August 1987
- journal article
- research article
- Published by Oxford University Press (OUP) in Plant Physiology
- Vol. 84 (4) , 1296-1300
- https://doi.org/10.1104/pp.84.4.1296
Abstract
Analysis of mesophyll protoplasts and cell wall extracts of leaf discs of Costa Rican wild lima bean (Phaseolus lunatus L.) shows that the linamarase activity is confined to the apoplast. Its substrate linamarin, together with the related enzyme hydroxynitrile lyase, is found inside the cells. This compartmentation prevents cyanogenesis from occurring in intact tissue, and suggests that linamarin has to be protected during any translocation across the linamarase rich apoplast.This publication has 14 references indexed in Scilit:
- Hevea Linamarase—A Nonspecific β-GlycosidasePlant Physiology, 1987
- Phloem Unloading of Amino Acids at the Site of Attachment of Cuscuta europaeaPlant Physiology, 1984
- Pathway of Phloem Unloading of Sucrose in Corn RootsPlant Physiology, 1983
- Subcellular Localization of Dhurrin β-Glucosidase and Hydroxynitrile Lyase in the Mesophyll Cells of Sorghum Leaf BladesPlant Physiology, 1981
- An Examination of Centrifugation as a Method of Extracting an Extracellular Solution from Peas, and Its Use for the Study of Indoleacetic Acid-induced GrowthPlant Physiology, 1980
- Energy-dependent Loading of Amino Acids and Sucrose into the Phloem of SoybeanPlant Physiology, 1979
- Tissue Distributions of Dhurrin and of Enzymes Involved in Its Metabolism in Leaves of Sorghum bicolorPlant Physiology, 1979
- Presence of the Cyanogenic Glucoside Dhurrin in Isolated Vacuoles from SorghumPlant Physiology, 1978
- The use of an abrasive in the isolation of cowpea leaf protoplasts which support the multiplication of cowpea mosaic virusVirology, 1975
- COPPER ENZYMES IN ISOLATED CHLOROPLASTS. POLYPHENOLOXIDASE IN BETA VULGARISPlant Physiology, 1949