Fast photoinduced electron transfer through DNA intercalation.
- 7 June 1994
- journal article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 91 (12) , 5315-5319
- https://doi.org/10.1073/pnas.91.12.5315
Abstract
We report evidence for fast photoinduced electron transfer mediated by the DNA helix that requires metal complexes that are avid intercalators of DNA. Here the donor bis(phenanthroline)(dipyridophenazine)ruthenium(II) [Ru(phen)2dppz2+] and acceptor bis(9,10-phenanthrenequinone diimine)(phenanthroline)rhodium(III) [Rh(phi)2phen3+] intercalate into DNA with Kb > 10(6) M-1. Luminescence quenching experiments in the presence of two different lengths of DNA yield upward-curving Stern-Volmer plots and the loss of luminescence intensity far exceeds the change in emission lifetimes. In the presence of a nonintercalative electron acceptor, Ru(NH3)3+(6), Ru(phen)2dppz2+ luminescence is quenched much less efficiently compared to that found for the intercalative Rh(phi)2phen3+ quencher and follows linear Stern-Volmer kinetics; steady-state and time-resolved Stern-Volmer plots are comparable in scale. These experiments are consistent with a model involving fast long-range electron transfer between intercalators through the DNA helix.Keywords
This publication has 17 references indexed in Scilit:
- Characterization of dipyridophenazine complexes of ruthenium(II): The light switch effect as a function of nucleic acid sequence and conformationBiochemistry, 1992
- Delineation of structural domains in eukaryotic 5S rRNA with a rhodium probeBiochemistry, 1992
- Recognition of tertiary structure in tRNAs by Rh(phen)2phi3+, a new reagent for RNA structure-function mappingBiochemistry, 1992
- Structural polymorphism in the major groove of a 5S RNA gene complements the zinc finger domains of transcription factor IIIA.Proceedings of the National Academy of Sciences, 1991
- PHOTOINDUCED ELECTRON TRANSFER QUENCHING OF EXCITED Ru(II) POLYPYRIDYLS BOUND TO DNA: THE ROLE OF THE NUCLEIC ACID DOUBLE HELIXPhotochemistry and Photobiology, 1991
- Luminescence of ruthenium(II) polypyridyls: evidence for intercalative binding to Z-DNA.1991
- Proton NMR studies of tris(phenanthroline) metal complexes bound to oligonucleotides: characterization of binding modesBiochemistry, 1990
- Accelerated Electron Transfer Between Metal Complexes Mediated by DNAScience, 1988
- Action at a Distance Along a DNAScience, 1988
- The Interactions of Ruthenium Hexaammine with Z-DNA: Crystal Structure of a RU(NH3)+36Salt of d(CGCGCG) at 1.2 Å ResolutionJournal of Biomolecular Structure and Dynamics, 1987