Separation of metal ions using a polymeric reversed-phase column and a Methylthymol Blue containing mobile phase

Abstract
A polystyrene–divinylbenzene reversed-phase column has been used in conjunction with a mobile phase containing methylthymol blue for the separation and visible detection of Mn2 +, Zn2 +, Cd2 + and Pb2 + in the presence of excess concentrations of matrix metals. Retention is due to the formation of a dynamically coated chelating stationary phase and is dependent upon mobile phase pH and ligand concentration. The method showed a different selectivity when compared to a pre-coated Methylthymol Blue chelating column and offers improved separation efficiency. Detection limits for Zn2 + and Pb2 + of 1 µM were achieved in solutions containing up to 1 M NaCl, following the post-column addition of a pH 5.9 buffer.

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