Effects of lithium and potassium on recovery of solute uptake capacity of Acer pseudoplatanus cells after gas‐shock

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Abstract
At concentrations of 10‐−3M, Li+ inhibits the recovery of solute uptake capacity of Acer pseudoplatanus L. cell suspension cultures after gas‐shock (i.e. after rapid exchange of the atmosphere in the culture flasks for ambient air). It also reduces solute uptake capacity of cells having already attained high rates of uptake during recovery from gas‐shock. The effects of Li+ are much greater in cells which have been cultivated in 7 mM K+ solution than in cells cultivated with higher K+ levels (19 mM). Increasing K+ concentration during recovery reverses the effect of 10–3M Li+ and, with sufficiently high concentrations of K+ (≥ 10‐−2M) during recovery, the solute uptake capacity of the fully recovered cells can even become greater than that of the control, at least for the low values of substrate concentration (here sulphate 10‐−5M). Since Li+ does not affect the time course of solute uptake measured over 15–20 min, it is thought that it interacts with the synthesis and turnover of the solute uptake machinery of the Acer pseudoplatanus cells. Thermodynamic analysis of the flux data also supports the hypothesis that Li+ inhibits the biosynthesis of specific sites of solute permeation, but it does not rule out the possibility that K+ interferes rather on the forces acting on the transport of the considered solutes than on the catalytic structures of permeation.