SEQUENTIAL TITRATION OF BOVINE LUNG AND SERUM ANTIBODIES AFTER PARENTERAL OR PULMONARY INOCULATION WITH PASTEURELLA-HAEMOLYTICA

  • 1 January 1979
    • journal article
    • research article
    • Vol. 40  (12) , 1690-1693
Abstract
Calves were vaccinated by intrabronchial or s.c. injection of formalinized P. haemolytica. Antibody [Ab] in serum, nasal washings and bronchoalveolar washings was titrated sequentially before and after calves were vaccinated and challenge exposed with live homologous bacteria. Bronchoalveolar washings were collected by fiberoptics bronchoscopy and Ab was titrated by indirect (antiglobulin) bacterial agglutination. Responsiveness to vaccination was related to initial serum Ab concentrations. Calves with serum Ab titers of 1:20 or more were non-responsive whereas with few exceptions, calves having titers of less than 1:20 responded to vaccination. Serum and lung Ab were apparently induced by s.c. or intrabronchial inoculation of formalinized P. haemolytica. By either route of immunization, serum Ab was more persistent than was lung Ab and pulmonary challenge exposure with live P. haemolytica did not alter existing titers.