EVALUATION OF 7SL RNA GENE SEQUENCES FOR THE IDENTIFICATION OF LEISHMANIA SPP.

1 April 2005

journal article
research article
Published by American Society of Tropical Medicine and Hygiene in The American Journal of Tropical Medicine and Hygiene

Vol. 72 (4) , 415-420
https://doi.org/10.4269/ajtmh.2005.72.415

Abstract

We evaluated the use of 7SL RNA gene sequences for the identification of Leishmania spp. A fragment (~137 basepairs) of the 7SL RNA gene from 13 reference strains and 18 clinical isolates of 11 different Leishmania species was amplified and sequenced using conserved primers. Reference strains from each Leishmania spp. complex showed unique sequences. The nucleotide sequences were compared pairwise and a range of 81.0–99.3% intercomplex similarity was observed. Clinical isolates of the same species had sequences identical to the corresponding reference strains; thus, the intraspecies similarity was 100%. A phylogenetic tree derived from the 7SL RNA gene partial sequences was constructed and is in agreement with accepted phylogenetic schemes.

Keywords

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The molecular evolution of trypanosomatidae
Published by Elsevier ,2004