Inter-Isolate Heterokaryosis in Pyricularia oryzae

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Abstract
Heterokaryons were synthesized between: monoconidial cultures, monoconidial cultures and field isolates, and field isolates of P. oryzae [rice blast pathogen]. Heterokaryotic hyphae appeared as a solid line of white mycelial tufts where contact occurred between colonies of compatible isolates when they were paired on an agar medium containing polished rice plus rose bengal. A mycelial fragment was taken from each of many tufts and allowed to grow. Hyphal tips from the resulting colonies provided cultures that were allowed to sporulate. Fifty or more monoconidial isolates were obtained from each hyphal tip isolate. Both parental types and some nonparental types were recovered in most instances. Thirteen of 20 different combinations involving 12 different field isolates produced heterokaryons. The percentage of heterokaryon 1.5-17.75. Heterokaryons also were formed between monoconidial progeny of 1 isolate (race IB-1, Texas) and field isolates. In addition, 7.6% of the crosses among 21 different field isolates produced tufts presumed to be heterokaryons.

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