Pancreatitis-Associated Ascitic Fluid: Effect on the Oxygen Consumption of Liver Cells

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Abstract
The effect of pancreatitis-associated ascitic fluid (PAAF) on the oxidative activity of rat liver cells was examined and a depression with 8 of 9 dog PAAF samples was found. Pancreatitis was induced in dogs by retrograde pancreatic duct infusion of bile salts plus trypsin. The resultant PAAF was collected, passed through a sterile 0.22-.mu. Micropore filter to remove bacteria and other debris and stored at -18.degree. until used. Liver cells were obtained from minced Sprague-Dawley rat livers by gentle homogenization and filtration through a nylon mesh filter to produce a cell suspension. The O2 utilization of liver cells incubated at 37.degree. with a 1:50 dilution of PAAF was compared to controls incubated with normal dog plasma. The O2 uptake of PAAF-treated cells was significantly lower with 7 of 9 ascitic fluids and the depression in O2 consumption was dose dependent over the range of 10-80 .mu.l PAAF. One human PAAF sample was tested and it caused a 72% decrease in liver cell O2 uptake as compared to controls. The observed depression in rat liver cell O2 uptake may be an expression of the cytotoxic component of PAAF that leads to cell damage and multiple organ failure in acute hemorrhagic pancreatitis.

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