Stable isotopically labeled internal standards in quantitative bioanalysis using liquid chromatography/mass spectrometry: necessity or not?
Top Cited Papers
- 12 January 2005
- journal article
- research article
- Published by Wiley in Rapid Communications in Mass Spectrometry
- Vol. 19 (3) , 401-407
- https://doi.org/10.1002/rcm.1790
Abstract
It appears to be a general belief that stable isotopically labeled (SIL) internal standards yield better assay performance results for quantitative bioanalytical liquid chromatography/mass spectrometry (LC/MS) assays than does any other internal standard. In this article we describe our experiences with structural analogues and SIL internal standards and their merits and demerits. SIL internal standards are the first choice, but deuterium‐labeled compounds may demonstrate unexpected behavior, such as different retention times or recoveries, than the analyte. In addition, a SIL internal standard with identical chemical properties as the analyte may cover up assay problems with stability, recovery, and ion suppression. Since SIL internal standards are not always available or are very expensive, structural analogues can be used, however, with consideration of several issues, which are usually displayed during method validation. Copyright © 2005 John Wiley & Sons, Ltd.Keywords
This publication has 13 references indexed in Scilit:
- Switching from an analogous to a stable isotopically labeled internal standard for the LC‐MS/MS quantitation of the novel anticancer drug Kahalalide F significantly improves assay performanceBiomedical Chromatography, 2004
- Quantitative analysis of D-24851, a novel anticancer agent, in human plasma and urine by liquid chromatography coupled with tandem mass spectrometryRapid Communications in Mass Spectrometry, 2004
- Quantitative analysis of the novel anticancer drug ABT‐518, a matrix metalloproteinase inhibitor, plus the screening of six metabolites in human plasma using high‐performance liquid chromatography coupled with electrospray tandem mass spectrometryJournal of Mass Spectrometry, 2004
- Quantitative analysis of ES‐285, an investigational marine anticancer drug, in human, mouse, rat, and dog plasma using coupled liquid chromatography and tandem mass spectrometryJournal of Mass Spectrometry, 2003
- Quantitative analysis of the novel depsipeptide anticancer drug Kahalalide F in human plasma by high‐performance liquid chromatography under basic conditions coupled to electrospray ionization tandem mass spectrometryJournal of Mass Spectrometry, 2002
- High-performance liquid chromatographic–tandem mass spectrometric evaluation and determination of stable isotope labeled analogs of rofecoxib in human plasma samples from oral bioavailability studiesJournal of Chromatography B, 2002
- LC-MS-MS experiences with internal standardsChromatographia, 2002
- Determination of pibutidine metabolites in human plasma by LC-MS/MSJournal of Pharmaceutical and Biomedical Analysis, 2000
- Effect of the sample matrix on the determination of indinavir in human urine by HPLC with turbo ion spray tandem mass spectrometric detectionJournal of Pharmaceutical and Biomedical Analysis, 1998
- Mass Spectrometry for Chemists and BiochemistsPublished by Cambridge University Press (CUP) ,1996