Alterations in the lenticular proteins of rats on riboflavin deficient diet

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Abstract
Effect of feeding riboflavin deficient diet to rats on lens protein composition was investigated. Total proteins and profile of soluble and insoluble proteins in lenses from rats fed on a riboflavin deficient diet for 7 wk were similar to that of the vitamin supplemented diet. Distribution of high MW protein (above 4 .times. 106 daltons) isolated from the 9900 g supernatant fraction was found to be significantly higher and .gamma. crystallin was significantly lower in riboflavin deficient group as compared to the normal lenses. The distribution of alpha and beta crystallins was not affected. Gel electrophoresis in sodium dodecyl sulfate of soluble lens proteins from riboflavin deficient animals had lower, proportions of polypeptide species with MW > 40,00 daltons while insoluble protein fraction had higher proportions of these polypeptide species as compared to control rats. Apparently, the composition of lens proteins is altered in riboflavin deficiency.