Microsomal Synthesis of Di- and Triacylglycerols in Rat Liver and Ehrlich Ascites Cells

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Abstract
The overall activity and specificity of the acyltransferases involved in triacylglycerol biosynthesis were compared in microsomes of normal rat liver and Ehrlich ascites cells using a variety of synthetic mono- and diacylglycerols of known structure as substrates. The ascites cell microsomes converted 2-monoacyl-sn-glycerols to diacylglycerols (25–67 nmol/h per milligram protein) at about two to three times the rate of liver microsomes (9–26 nmol/h per milligram protein). They yielded two to three times more 2,3-diacyl-sn-glycerols (15–26%) than did liver microsomes (6–12%), although the major product of acylation of the 2-monoacyl-sn-glycerol was the 1,2-isomer (73–85%). The microsomes of the ascites cells converted two to three times as much 2,3-diacyl-sn-glycerol to triacylglycerol as did liver microsomes, although both tissues acylated 1,2-diacyl-sn-glycerols preferentially (58–85%). It is suggested that the ascites cells may have reverted to a more primitive stage of lipid metabolism characterized by the ability of direct esterification of the products of lipase activity.

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