Rapid Preparation of Thick Sections of Fleshy Plant Tissues
- 1 January 1952
- journal article
- research article
- Published by Taylor & Francis in Stain Technology
- Vol. 27 (1) , 29-35
- https://doi.org/10.3109/10520295209105057
Abstract
Methods are described for permanent micro-slide preparations of soft, large-celled plant tissues such as ripe fruit. Thick sections (200–800 [t) cut on a sliding microtome are aspirated in an aqueous killing agent; after fixing and washing, the sections are dehydrated and cleared in an alcohol-xylene series. Infiltration with 20, 30, and 40% solutions of mountant prior to mounting the sections is necessary to avoid too abrupt changes in the cleared tissues. Several staining methods have been successfully used for different purposes. The final preparations showed nearly perfect preservation of intact cells and intercellular spaces in their 3-dimension-al structure.Keywords
This publication has 4 references indexed in Scilit:
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- Staining of plant Materials Cleared in NaOHStain Technology, 1949
- An Adaptable Staining Schedule for Plant TissuesStain Technology, 1948
- Vacuoles in primary meristemsCell and tissue research, 1932