Timing of swarmer cell cycle morphogenesis and macromolecular synthesis by Hyphomicrobium neptunium in synchronous culture

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Abstract
The swarmer cycle of H. neptunium consists of a temporal sequence of discrete developmental events. To time morphogenesis and to investigate modulations in macromolecular synthesis, methods for synchronous culture were attempted. During synchrony, swarmer maturation occurred over 32%, hyphal growth occurred over 36%, and bud maturation occurred over 32% of the time required to complete the swarmer cycle. Daughter cells were released after 265 min. DNA replication was discontinuous, having a G1 period of approximately 180 min. In addition, RNA and protein syntheses were depressed during the earlier phases of development.