An exposed tyrosine residue of RNase T1 and its involvement in the interaction with guanylic acid
Open Access
- 23 September 1985
- journal article
- Published by Wiley in FEBS Letters
- Vol. 189 (2) , 167-170
- https://doi.org/10.1016/0014-5793(85)81017-6
Abstract
A photo‐CIDNP spectrum of RNase T1 showed that 4 out of the total 9 tyrosines are accessible to the photosensitive dye, while none of the histidine and tryptophan residues are accessible. By comparison with the results of nitration of tyrosine side chains followed by peptide analysis, it can be concluded that Tyr 45 is mostly exposed on the surface of RNase T1. On addition of 2'‐GMP, the signal of Tyr 45 shifts upfield and is remarkably broadened, which suggests that the phenyl ring of Tyr 45 stacks on the guanine ring of 2'‐GMP. Similar phenomena were observed on addition of 3'‐GMP and 3'‐dGMP.Keywords
This publication has 13 references indexed in Scilit:
- pH‐induced change in nucleotide binding geometry in the ribonuclease T1‐2'‐guanylic acid complexFEBS Letters, 1985
- Binding modes of inhibitors to ribonuclease T1 as studied by nuclear magnetic resonanceBiochemistry, 1985
- Isocratic Separation of PTH-Amino Acids at Picomole Level by Reverse-Phase HPLC in the Presence of Sodium Dodecylsulfate1The Journal of Biochemistry, 1985
- Chemically induced dynamic nuclear polarization studies of guanosine in nucleotides, dinucleotides, and oligonucleotidesBiochemistry, 1984
- Specific protein-nucleic acid recognition in ribonuclease T1–2′-guanylic acid complex: an X-ray studyNature, 1982
- A 15N-NMR Study on Ribonuclease T1-Guanylic Acid ComplexThe Journal of Biochemistry, 1982
- Photo-CIDNP Studies of ProteinsPublished by Springer Nature ,1982
- The Aromatic Residues of Bovine Pancreatic Ribonuclease Studied by 1H Nuclear Magnetic ResonanceEuropean Journal of Biochemistry, 1979
- Proton and phosphorus nuclear magnetic resonance studies of ribonuclease T1Biochemistry, 1979
- The Structure and Function of Ribonuclease T1: XVII. Isolation and Amino Acid Sequences of Papain and Subtilisin Peptides from Ribonuclease T1—The Complete Covalent Structure of Ribonuclease T1*The Journal of Biochemistry, 1971