Direct Visualization of the Putative Portal in the Kaposi's Sarcoma-Associated Herpesvirus Capsid by Cryoelectron Tomography
- 1 April 2007
- journal article
- Published by American Society for Microbiology in Journal of Virology
- Vol. 81 (7) , 3640-3644
- https://doi.org/10.1128/jvi.02254-06
Abstract
Genetic and biochemical studies have suggested the existence of a bacteriophage-like, DNA-packaging/ejecting portal complex in herpesviruses capsids, but its arrangement remained unknown. Here, we report the first visualization of a unique vertex in the Kaposi's sarcoma-associated herpesvirus (KSHV) capsid by cryoelectron tomography, thus providing direct structural evidence for the existence of a portal complex in a gammaherpesvirus. This putative KSHV portal is an internally localized, umbilicated structure and lacks all of the external machineries characteristic of portals in DNA bacteriophages.Keywords
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