Distribution of 7-methylguanine and of replication sites in the different kinetic classes of DNA from rats treated with dimethyhtitrosamine
- 1 January 1978
- journal article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 5 (9) , 3357-3364
- https://doi.org/10.1093/nar/5.9.3357
Abstract
The distribution of 7-methylguanine in the families of repetitive and unique sequences of rat liver chromatin DNA has been studied using the technique of DNA-DNA reassociation. Rats were injected with di[14C]methylnitrosamine and chromatin DNA was prepared 3 h later. The distribution of 7-methylguanine was found to be random between these classes of DNA. We have also studied chromatin DNA from rats treated with unlabelled DMN plus [3H]thymidine in this way, in order to find if DMN affects DNA synthesis within any one kinetic class. Our results suggest that there is no difference in the extent of synthesis between these classes.Keywords
This publication has 18 references indexed in Scilit:
- Distribution of covalently bound benzo(a)pyrene in chromatinBiochemical and Biophysical Research Communications, 1977
- Non-random binding of a chemical carcinogen to the DNA in chromatinBiochemical and Biophysical Research Communications, 1977
- Formation and Metabolism of Alkylated Nucleosides: Possible Role in Carcinogenesis by Nitroso Compounds and Alkylating AgentsPublished by Elsevier ,1977
- Nonuniform distribution of DNA repair in chromatin after treatment with methyl methanesulfonateNucleic Acids Research, 1977
- Distribution along DNA of the bound carcinogen N-acetoxy-N-2-acetylaminofluorene in chromatin modified in vitroChemico-Biological Interactions, 1976
- Repair replication of DNA in the intact animal following treatment with dimethylnitrosamine and with methyl methanesulphonate, studied by fractionation of nuclei in a zonal centrifugeBiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1976
- Reduced DNA repair in mouse satellite DNA after treatment with methyl-methanesulfonate, and N-methyl-N-nitrosoureaNucleic Acids Research, 1976
- A micro-biuret method for estimating proteinsAnalytical Biochemistry, 1964
- The precision of ultraviolet absorption measurements in the Schmidt-Thannhauser procedure for nucleic acid estimationBiochimica et Biophysica Acta, 1962
- PROTEIN MEASUREMENT WITH THE FOLIN PHENOL REAGENTJournal of Biological Chemistry, 1951