Induction and Repair of DNA Strand Breaks in Cultured Mammalian Cells Following Fast Neutron Irradiation
- 1 January 1979
- journal article
- research article
- Published by Taylor & Francis in International Journal of Radiation Biology
- Vol. 36 (6) , 639-648
- https://doi.org/10.1080/09553007914551471
Abstract
Induction and repair of DNA breaks following irradiation with NIRS cyclotron neutrons were studied in cultured mammalian cells (mouse lymphoma L5178Y) in comparison to those following .gamma.-rays. The yield of the total single-strand breaks, 3''OH terminals and sites susceptible to S1 endonuclease following fast neutrons was 50% of that following .gamma.-irradiation. The yield of double-strand breaks was slightly higher after fast neutrons than after .gamma.-rays. The percentage of the total single-strand breaks remaining unrejoined at 3 h after post-irradiation incubation was distinctly higher after the fast neutrons than after .gamma.-rays. The neutron-induced damage appears to carry a higher proportion of alkali-labile lesions compared to .gamma.-rays. The increase in the yield of double-strand breaks and of unrejoinable breaks is responsible for a high rbe [relative biological effectiveness] of the cyclotron neutrons.This publication has 19 references indexed in Scilit:
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