SdrX, a Serine-Aspartate Repeat Protein Expressed by Staphylococcus capitis with Collagen VI Binding Activity
- 1 November 2004
- journal article
- Published by American Society for Microbiology in Infection and Immunity
- Vol. 72 (11) , 6237-44
- https://doi.org/10.1128/iai.72.11.6237-6244.2004
Abstract
Staphylococcus capitis ( S. capitis ) has been implicated in a large proportion of coagulase-negative staphylococcal infections in very-low-birth-weight infants. To identify potential therapeutic targets, the S. capitis genome was probed for the presence of genes encoding microbial surface components recognizing adhesive matrix molecules (MSCRAMM). By using Southern blot analysis, an S. capitis gene, designated sdrX , that contained sequence motifs consistent with the Sdr family of MSCRAMM proteins was identified. By using monospecific antisera in Western blot and flow cytometry, SdrX was demonstrated to be expressed on the surface of S. capitis. Human collagen type VI was found to bind both the recombinant A domain of SdrX and viable S. capitis expressing SdrX. SdrX is the first collagen-binding Sdr protein described and is the first MSCRAMM protein identified in S. capitis .Keywords
This publication has 31 references indexed in Scilit:
- Microbial adhesins recognizing extracellular matrix macromoleculesPublished by Elsevier ,2004
- Anti-Clumping Factor A Immunoglobulin Reduces the Duration of Methicillin-ResistantStaphylococcus aureusBacteremia in an Experimental Model of Infective EndocarditisAntimicrobial Agents and Chemotherapy, 2003
- Nosocomial Spread of a Staphylococcus capitis Strain with Heteroresistance to Vancomycin in a Neonatal Intensive Care UnitJournal of Clinical Microbiology, 2002
- Staphylococcus caprae Strains Carry Determinants Known To Be Involved in Pathogenicity: a Gene Encoding an Autolysin-Binding Fibronectin and the ica Operon Involved in Biofilm FormationInfection and Immunity, 2001
- Staphylococcus aureus Sortase, an Enzyme that Anchors Surface Proteins to the Cell WallScience, 1999
- Binding of the NG2 Proteoglycan to Type VI Collagen and Other Extracellular Matrix MoleculesJournal of Biological Chemistry, 1996
- Type VI collagen mutations in Bethlem myopathy, an autosomal dominant myopathy with contracturesNature Genetics, 1996
- Integrin and Arg-Gly-Asp Dependence of Cell Adhesion to the Native and Unfolded Triple Helix of Collagen Type VIExperimental Cell Research, 1993
- Structural and functional features of the .alpha.3 chain indicate a bridging role for chicken collagen VI in connective tissuesBiochemistry, 1990
- Structure and Macromolecular Organization of Type VI CollagenaAnnals of the New York Academy of Sciences, 1985