New Method for the Rapid Quantitation of Immunofluorescence 2
- 1 July 1973
- journal article
- research article
- Published by Oxford University Press (OUP) in JNCI Journal of the National Cancer Institute
- Vol. 51 (1) , 159-163
- https://doi.org/10.1093/jnci/51.1.159
Abstract
A rapid, inexpensive, and reliable method for quantitating either antibody and membrane or cytoplasmic fluorescence was described. The method compared the fluorescence of a single cell with a reference slit, illuminated directly from the dark-field condenser by glass fiber optics. The brightness of the reference slit could be altered by rotation of a continuously variable neutral-density filter. Some applications of the method to the study of distribution, expression, and cellular localization of tumor-related antigens were described.Keywords
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