Real-Time PCR for Detection and Differentiation of Gram-Positive and Gram-Negative Bacteria
Open Access
- 1 November 2002
- journal article
- research article
- Published by American Society for Microbiology in Journal of Clinical Microbiology
- Vol. 40 (11) , 4304-4307
- https://doi.org/10.1128/jcm.40.11.4304-4307.2002
Abstract
We developed a consensus real-time PCR protocol that enables us to detect spiked bacterial 16S DNA from specimens such as water, urine, plasma, and sputum. The technique allows an exact Gram stain classification of 17 intensive care unit-relevant bacteria by means of fluorescence hybridization probes. All tested bacteria were identified correctly, and none gave a false-positive signal with the opposite Gram probe.Keywords
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