Complementation mapping in microcell hybrids: Localization ofFpgs andAk-1 onmus musculus chromosome 2
- 1 January 1983
- journal article
- research article
- Published by Springer Nature in Somatic Cell and Molecular Genetics
- Vol. 9 (1) , 69-84
- https://doi.org/10.1007/bf01544049
Abstract
The gene encoding folylpolyglutamyl synthetase (FPGS) was assigned to mouse chromosome 2 by complementation mapping. Chinese hamster ovary cells (AuxBl) deficient in FPGS, and consequently auxotrophic for glycine, adenosine, and thymidine (gat−), were employed as recipients in microcell-mediated chromosome transfer experiments. Mouse chromosomes derived from diploid embryo fibroblasts were introduced into hamster AuxBl cells, and gat+ microcell hybrids were selected in medium lacking adenosine and thymidine. Mouse chromosome 2 was the only donor chromosome whose presence correlated with expression of FPGS activity. Furthermore, every gat+ hybrid clone expressed murine AK-1, a marker previously assigned to chromosome 2. Eight of 20 clones analyzed retained deletion chromosomes derived from mouse chromosome 2. These clones were used to localize murine Fpgs and Ak-1 to a region of this chromosome, namely 2(cen→C1).This publication has 39 references indexed in Scilit:
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