Investigations on the Emergence of Larvae from the Cysts of the Potato-root Eelworm, Heterodera rostochiensis.

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Abstract
The foregoing results throw light on the conditions under which hatching tests can be carried out. In the first place, pre-soaking cysts in water for a period of 10 days increases the rate of larval emergence but has no effect on the total number of hatchable larvae. Increase in temperature has a similar effect up to 25°C, but at 80°C. hatching is virtually inhibited. Volume of diffusate per cyst is without effect either on the number of hatchable larvae or on their rate of hatching. Neither was any effect observed from alterations in pH between 8.2 and 8.1. The effect of dilution is more or less linear when dilution is plotted geometrically against cither the number of hatchable larvae or the latter expressed as a proportion of the original cyst contents and subjected to angular transformation. For the effect to be appreciable, dilution must be of a high order. Dilution to a half or a quarter of the original concentration has little appreciable effect. The effect of dilution on the rate of hatching is not obvious and the variations in it resulting from dilution are probably random. Direct sunlight inhibits hatching, diffused daylight is without effect as compared with darkness ; cysts exposed to the action of sunlight and diffusate do not hatch when later removed to the dark. It appears reasonable to assume that this may be due to some lethal effect.