Regulation of hydrolase formation and phosphate release in turimycin fermentations

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Abstract
During the turimycin fermentation hydrolytic enzymes are excreted responsible for orthophosphate release from phosphate-containing dissolved and undissolved complex medium constituents. Following a phosphate-limited growth period the phosphate release leads to a second growth period (diauxic growth). Depending on the rate of phosphate release the length of the lag phase of diauxic growth changes in different fermentations. The resulting second growth period is correlated with a transient delay in the formation of turimycin, of phosphatases and of nucleases. The amylolytic activities are formed already within the first hours after the beginning of fermentations. Phosphatases, nucleases and protease are excreted parallel to turimycin formation after extracellular phosphate limitation in presence of ammonia and glucose. A special role of phosphate limitation initiating enzyme synthesis is proposed.