Induction of a Host-specific Chromatin-associated Glycopolypeptide by Human Cytomegalovirus

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Abstract
Analysis of chromatin preparations from [3H]glucosamine-labeled human foreskin fibroblasts revealed that a chromatin-associated glycopolypeptide with the approximate MW 130,000 (130K) is induced in response to either infection with human cytomegalovirus (HCMV) or serum treatment. Comparative limited proteolysis suggested that the [3H]glucosamine-labeled 130K polypeptides induced by these different stimuli were not identical. This observation was in contrast to results obtained by immunoprecipitation with antisera raised against the 130K glycopolypeptide from serum-induced cells which favored a relatedness to the 130K polypeptide from virus-infected cultures. Two-dimensional separation by isoelectric focusing and sodium dodecyl sulfate-polyacrylamide gel electrophoresis subsequently showed that the 130K glycopolypeptide from serum-induced cells consists of 2 components, one of which is identical to the single component observed in samples from HCMV-infected cultures. Experiments on the effect of glycosylation inhibitors on DNA replication in HCMV-infected as well as in serum-induced cells support the view that the host-specific chromatin-associated glycopolypeptide may be involved in DNA replication in infected cells.