A Rapid Screening Slide Test for Hemoglobin S

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Abstract
A slide method for hemoglobin S detection is described. A blood film is flooded with a solution of sodium dithionite and 2.19 M phosphate buffer. Hemoglobin S is reduced and rendered insoluble whereas other hemoglobins are eluted. The presence or absence of hemoglobin pigment is apparent grossly and microscopically. The slide method was compared to the metabisulfite and electrophoretic methods in 103 Negro patients. Thirty-five patients had erythrocytes containing hemoglobin S. The slide test failed to detect hemoglobin S in only one infant with an FS electrophoretic pattern. No false-positives were encountered. The major advantages of the slide method are speed of preparation, ease of interpretation, and preservation of color and morphologic characteristics on storage.

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