Inhibition of degranulation of human polymorphonuclear leukocytes by complement factor D

11 September 1995

journal article
Published by Wiley in FEBS Letters

Vol. 371 (3) , 300-302
https://doi.org/10.1016/0014-5793(95)00926-z

Abstract

A degranulation inhibiting protein could be isolated from human plasma ultrafiltrate by a three‐step purification method including ion‐exchange chromatography, gelfiltration and affinity‐chromatography. The protein was identified as complement factor D by means of sequence analysis. Its degranulation inhibiting activity was determined with regard to its effect on the FNLPNTL‐induced lactoferrin secretion of human polymorphonuclear leukocytes. Complement factor D caused a dose‐dependent decrease of the FNLPNTL‐stimulated lactoferrin degranulation down to 34% of stimulated controls.

Keywords

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