Two-photon laser scanning fluorescence microscopy using photonic crystal fiber
- 1 January 2004
- journal article
- research article
- Published by SPIE-Intl Soc Optical Eng in Journal of Biomedical Optics
- Vol. 9 (5) , 922-927
- https://doi.org/10.1117/1.1778734
Abstract
We report the application of a simple yet powerful modular pulse compression system based on photonic crystal fibers that improves on incumbent two-photon laser scanning fluorescence microscopy techniques. This system provides more than a sevenfold increase in fluorescence yield when compared with a commercial two-photon microscopy system. From this, we infer pulses of IR radiaton of less than 35 fs duration reaching the sample. © 2004 Society of Photo-Optical Instrumentation Engineers.Keywords
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