Abstract
A new method of differentiating safranin in pollen-mother-cell smears and paraffin sections is described in detail. Slides stained in safranin are dehydrated in a series of alcoholic solutions containing 1.5% picric acid with constantly decreasing percentages of water. Differentiation is principally effected in 83% alcohol containing 1.5% picric acid and completed in the final dehydration and clearing. Counterstains may be applied in clove oil if desired.

This publication has 3 references indexed in Scilit: