Dermatan Sulfate-Chondroitin Sulfate Copolymers from Umbilical Cord. Isolation and Characterization

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Abstract
Dermatan sulfate-chondroitin sulfate copolymers have been isolated from human umbilical cord as a major galactosaminoglycan component of this issue. The galactosaminoglycan fraction was obtained from this tissue by papain [EC 3.4. 22.2] digestion followed by precipitation with cetylpyridinium chloride in a yield of 700 mg per 100 g of dry tissue. Ethanol fractionation resolved 4–5 subfractions differing in relative content of L-iduronic acid and D-glucuronic acid. No galactosaminoglycan containing either solely L-iduronic acid or D-glucuronic acid was obtained. The copolymeric structure of the material in each subfraction was demonstrated by analysis of oligosaccharide fragments obtained by chondroitinase-AC [EC 4.2.2.5] digestion. All the polymers contained repeating disaccharide units, D-glucuronosyl-N-acetyl-gal-actosamine D-glucuronosyl-N-acetygalactosamine 4-sulfate, of which D-glucuronosy-N-acetyl-gactosamine 6-sulfate and L-iduronosy-N-acetylgalactosamine 4-sulfate were predominant. Both iduronic acid- and glucuronic acid-containing units were arranged in clusters. The presence of a considerable amount of nonsulfated disaccharide units was noted. The copolymers show extensive polydispersity in elec-trophoresis on cellulose acetate and gel chromatography on Sephadex G-200.

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