The Mode of Action of Antibodies which Destroy Factor VIII

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Abstract
Summary. In 1959 Biggs & Bidwell proposed the hypothesis that the reaction between antibody and factor VIII could be interpreted as a second‐order reaction and that in antibody excess the amount of antibody present could be measured using a linear calibration graph relating the logarithm of residual factor VIII to antibody concentration after incubation for a fixed period of time. This hypothesis has been re‐examined because antibody is seldom present in excess in most routine test systems and in view of a new and attractive hypothesis put forward by Pool & Miller (1972). All the evidence seems to suggest that the original hypothesis of Biggs & Bidwell is adequate for assessing the potency of antibodies of the type occurring in many haemophilic patients.