INDUCTION OF ACUTE-PHASE SERUM-PROTEIN SAA REQUIRES BOTH RNA AND PROTEIN-SYNTHESIS

Google Scholar
  • 1 January 1978
    • journal article
    • research article
    • Vol. 59  (3) , 305-310
Abstract
SAA is a normal acute-phase serum protein which was identified by its cross-reaction with antibodies to the amyloid A fibril protein, AA, i.e., associated with secondary amyloidosis. The induction of SAA [in mice] by bacterial lipopolysaccharide (LPS) was studied with 3 inhibitors of protein synthesis: cycloheximide, actinomycin D and galactosamine. Each of the 3 agents when administered simultaneously with LPS completely abolished induction of SAA for at least 6 h. They were all significantly effective when given 1.5 h after LPS but by 3 h after LPS the inhibitory effect of actinomycin D on SAA induction was markedly reduced. Cycloheximide alone induced significant concentrations of SAA, but a longer time was required than for LPS. Apparently the acute-phase SAA response is characterized by both RNA and protein synthesis which is initiated by the acute-phase inducing agent and precedes the appearance of elevated SAA concentrations in the serum.