SOME HISTOCHEMICAL OBSERVATIONS ON NORMAL AND DISEASED HUMAN SKELETAL MUSCLE

Open Access
Publisher Website
Google Scholar
Abstract
Biopsy samples of muscle were taken from cases of a wide variety of muscular and neuromuscular disorders. The results obtained using techniques to demonstrate sulfydryl and disulfide groups, amino groups, and also using Gomori''s aldehyde-fuchsin stain were compared with those observed in normal human muscle taken either at biopsy or post mortem. Sulfur linkages seem to be present as sulfydryl groups in the myofibrils and in the neurokeratin network of nerves, and their distribution is essentially similar to that for amino groups. There is no change in concentration of either of these groupings in any disease* studied, even when histological changes are severe. There may sometimes be an increase in elastic fibers in diseased muscle, but this is not associated with any particular diagnosis.