Cultivation in Tissue-Culture of Cytopathogenic Agent from Bovine Mucosal Disease.

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Abstract
The isolation and cultivation in tissue culture of a cytopathogenie agent recovered from tissues of cattle with mucosal disease from 2 separate herds is reported. A homologous tissue culture system was used with bovine embryonic kidney cells and newborn, antibody-free calf serum. The virus was inactivated by temper atures above 50[degree]C for 15 minutes, would pass an 03 Selas filter and had and 11th passage titer of 10-5. In sheep, the virus stimulated the formation of neutralizing antibodies. A preliminary neutralization study indicated that antibodies are common in animals from herds having no previous history of mucosal disease.

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