Technic in the Study of Vascular Systems in Plants

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Abstract
Methods of studying the morphology of rosace- ous fruits (1) dissect the buds and immerse over night in 20% HC1; then in 5% NaOCl until bleached; after dehydrating, clearing and mounting in cedar oil, examine them in dark field or with intense reflected light: (2) place the living stems, bearing buds, in a 0.01% aq. soln. of Niagara sky blue, forcing infiltration of the dye by means of pressure obtained in a pressure cooker with a bicycle pump; the buds are dehydrated and embedded by ordinary methods. Photographs may be taken of the cut surfaces of such a stained bud, after each section has been removed from the block.

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